Gene transfer Technology

Gene Transfer Technologies

Genetic Engineering is a process that alters the genetic structure of an organism by either 

removing or introducing DNA. Genetic engineering takes the gene directly from one organism 

and inserts it in the other for the purpose of changing its characteristics. Genetic engineering 

also called genetic modification or genetic manipulation.

Gene transfer is to transfer a gene from one DNA molecule to another DNA molecule.

 The directed desirable gene transfer from one organism to another and the subsequent 

stable integration & expression of foreign gene into the genome is referred as genetic 

transformation.

 The transferred gene is known as transgene and the organism that develop after a 

successful gene transfer is known as transgenic.

Methods Of Gene Transfer

A. DNA Transfer by Natural Methods:

1. Conjugation

2. Bacterial Transformation

3. Retroviral Transduction

B. DNA TRANSFER BY ARTIFICIAL METHOD:

1. Microinjection (Physical Method)

2. Biolistics Transformation (Physical Method)

3. DNA transfer by calcium phosphate method (Chemical Method)

4. Liposome mediated transfer (Chemical Method)

5. Electroporation (Electrical Method).